Modified staining protocol with Safranin O and Astra Blue for the plant histology

Many staining protocols are widely applied in botanical microtechniques and serve specific histological purposes. In particular, some dyes used simultaneously to receive contrasting colorations of different chemical structures, e.g., lignin cellulose. One the most popular differential is based on Safranin O / Astra Blue combination. a water-soluble basic dye that stains red. also but having an acidic reaction, which cellulose blue. Usually, 1–2 % solution distilled water or 50–70 ethanol combination with 0.5–1 detect lignified structures obtain pictures convenient for light microscopy. For long time, was exclusively solutions, such recommendation without additional options indicated producers’ web sites. However, 2002 it proposed use 1 95 identify tissues. Later, successfully by other researchers experimental purposes.We tested modified protocol application both solutions slightly lower concentration (70 %) flower buds Gagea lutea (Liliaceae) found working well. We believe these two 70 allows simplifying procedure plant material due same concentrations dissolvent reducing difference solvent between following solutions. Such can be effectively histology purposes, especially where there need distinguish secretory